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KMID : 0545120080180071274
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Journal of Microbiology and Biotechnology
2008 Volume.18 No. 7 p.1274 ~ p.1277
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Inactivation of Listeria monocytogenes in Brine and Saline by Alternating High-Voltage Pulsed Current
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Lee Mi-Hee
Han Dong-Wook
Woo Yeon I.
Uzawa Masakazu
Park Jong-Chul
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Abstract
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The inactivating efficiency of alternating high-voltage pulsed (AHVP) current was investigated in brine (20 w/v% NaCl) and saline (0.9 w/v% NaCl) inoculated with 1× 107 cells/ml of Listeria monocytogenes. AHVP current at 12 V with 1 pulse completely inactivated L. monocytogenes in brine within 3 ms, while the bacteria in saline were fully inactivated by 10-pulsed electric treatment at 12 V within the same time. Electron microscopic observation demonstrated substantial structural damage of electrically treated L. monocytogenes in brine. These results suggest that AHVP treatment would be effective for the rapid and complete inactivation of L. monocytogenes in brine or saline solution.
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KEYWORD
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Listeria monocytogenes, alternating high-voltage pulsed current, brine, saline, inactivation
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